Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

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Summary: A resonant-scanning karate goju ryu book multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate.In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed.We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process.For complete details jumbo pe?ete yüzü?ü on the use and execution of this protocol, please refer to Borah et al.

(2021).